WHAT IS NEW IN CONSED 17.0 Solexa and 454 reads are now fully supported. Solexa reads Millions of Solexa reads can be aligned to human-genome sized reference sequences and smaller selected regions viewed in consed. Consed can handle millions of such reads with a chromosome-sized reference sequence with reasonable response times. Reports on variants can be produced or these regions can be conveniently navigated in consed. Similarly with regions of high (or low) depth of coverage. 454 reads are now fully supported, both as part of Newbler assemblies, and when aligned to a reference sequence. Problems with sff2scf and bringing up the traces are fixed. Cross_match (available separately from Consed-see below) This familiar gapped alignment tool is now so fast that it can be used as a genome search engine. For example, a million solexa reads can be searched against the entire (repeat masked) human genome in less than an hour for most desktop computers and datasets. Consed-ready ace files can be prepared in a fraction more time. Peak memory usage: 4 to 5 Gb. Reports sequence variants & confirmed segments and strength of evidence, for resequencing applications Local, Global, Semi-global, and spliced (RNAseq) alignments Flexible reporting of hits: all, single top, all top, or top if sufficiently better than others